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Synthesis of Eicosapentaenoic Acid-enriched Phosphatidylcholine and its Effect on Pro-inflammatory Cytokine Expression


Jae Yeul Baek, Eun Na and Sun Young Lim*   Pages 1 - 6 ( 6 )


Aim and Objective: We synthesized eicosapentaenoic acid-enriched phosphatidylcholine (EPA-PC) and investigated its effect on the production of lipopolysaccharide (LPS)-induced cytokines in murine splenocytes.

Material and Methods: The culture supernatants of splenocytes, which was exposed to EPA-PC along with LPS, was harvested to determine the production of cytokines [interleukin (IL)-4 , IL-5, IL-6, interferon (IFN)-γ, IL-2 and IL-12/IL-23(p40)]. Cytokines were measured using enzyme-linked immunosorbent assay (ELISA).

Results: The co-administration of EPA-PC with LPS resulted in a significantly lower IFN-γ expression than that observed with LPS alone (p < 0.01). Moreover, treatment with EPA-PC and LPS significantly decreased IL-2, IL-6 and IL-12/IL-23(p40) expression (p < 0.01). Co-administration of EPA-PC at a concentration of 0.3 μg/mL with LPS resulted in a higher IL-5 expression after 24 hr of treatment when compared to LPS alone (p < 0.05).

Conclusion: These results suggest that EPA-PC is more effective in decreasing the expression of pro-inflammatory cytokines [IL-2, IFN-γ, IL-6 and IL-12/IL-23(p40)] upon induction of inflammation.


Eicosapentaenoic acid, phosphatidylcholine, cytokines


Division of Marine Bioscience, Korea Maritime and Ocean University, Busan, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan, Division of Marine Bioscience, Korea Maritime and Ocean University, Busan

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