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A Decade of Yeast Surface Display Technology: Where Are We Now?

[ Vol. 11 , Issue. 2 ]

Author(s):

Eric V. Shusta, Lauren R. Pepper, Yong Ku Cho and Eric T. Boder   Pages 127 - 134 ( 8 )

Abstract:


Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Recent advances have greatly expanded the capability of yeast surface display, and are highlighted by cellbased selections, epitope mapping, cDNA library screening, and cell adhesion engineering. In this review, we discuss the state-of-the-art yeast display methodologies and the rapidly expanding set of applications afforded by this technology.

Keywords:

Yeast surface display, protein engineering, library screening

Affiliation:

Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.



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