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Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® – Mass Spectrometry (RF-MS)

[ Vol. 12 , Issue. 8 ]

Author(s):

Maxine Jonas, William A. LaMarr and Can Ozbal   Pages 752 - 759 ( 8 )

Abstract:


In this review various technologies and approaches for the utilization of mass spectrometry in high throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. The RapidFire mass spectrometry system, an integrated on-line solid-phase extraction system interfaced to a triple-quadrupole mass spectrometer is described in detail, and the identification of a series of inhibitors of the acetyl-coenzyme A carboxylase (ACC) assay is described.

Keywords:

High throughput screening, acetyl-coenzyme A carboxylase, RapidFire, mass spectrometry

Affiliation:

BioTrove, Inc., 12 Gill Street, Suite 4000, Woburn, MA 01801, USA.



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