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Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization

[ Vol. 12 , Issue. 8 ]

Author(s):

Joy Concepcion, Krista Witte, Charles Wartchow, Sae Choo, Danfeng Yao, Henrik Persson, Jing Wei, Pu Li, Bettina Heidecker, Weilei Ma, Ram Varma, Lian-She Zhao, Donald Perillat, Greg Carricato, Michael Recknor, Kevin Du, Huddee Ho, Tim Ellis, Juan Gamez, Michael Howes, Janette Phi-Wilson, Scott Lockard, Robert Zuk and Hong Tan   Pages 791 - 800 ( 10 )

Abstract:


The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.

, Label-Free, Detection, of, Biomolecular, Interactions, Using, BioLayer, Interferometry, for, Kinetic, Characterization

Affiliation:

ForteBio, Inc., 1360 Willow Road Suite 201, Menlo Park, CA 94025, USA.



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